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Useful validation of sorted fractions was executed by xenotransplantation, the results of which was combined with miRNA expression profiling to make stem cell-relevant miRNA expression profiles.
To determine whether or not miRNA are differentially expressed in LSC and HSC, we fractionated sixteen AML individual samples and a few lineage-depleted (Lin–) cord blood (CB) samples working with CD34 and CD38 into four populations and subjected Each individual to world-wide miRNA profiling; the stem mobile content material of every portion was functionally assayed by xenotransplantation (Figures 1A and S1A).
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(J) Proliferation measured by BrdU incorporation assay of CTRL or 126KD transduced 8227 cells in vitro.
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The LSC frequency was reduced in two of three samples on 126KD (Figures 5C and S5D). Alongside one another, these conclusions advise that 126KD provides heterogeneous responses with LSC functionality and frequency lessened inside of a subset of AML people.
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(D) Agent intracellular movement plots for the detection of CDK3 and pRB Ser807/811. Graph down below represents a few impartial intracellular movement experiments for every problem exactly where the necessarily mean fluorescence intensity was compared. Signify ± SEM; ∗p=
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